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IL1β Rabbit pAb (A11369)

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ABclonal:Western blot - IL1β Rabbit pAb (A11369)

Western blot analysis of extracts of various cell lines, using IL1β antibody (A11369) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for IL1β Rabbit pAb(A11369)
Review
ABclonal: review for IL1β Rabbit pAb(A11369)

Overview

Product nameIL1β Rabbit pAb
Catalog No.A11369
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 124-223 of human IL1β (NP_000567.1).
SequenceCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEIN
Gene ID3553
Swiss protP01584
SynonymsIL-1; IL1-BETA; IL1F2; IL1 beta; IL1B
Calculated MW30kDa
Observed MW17kDa/30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesTHP-1, A-431, A-549, Mouse liver, Mouse spleen, Rat liver, Rat spleen
Cellular locationCytoplasm, Cytoplasmic vesicle, Lysosome, Secreted, autophagosome, cytosol, exosome
Customer validation

(Mouse colon cancer cells)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - IL1β Rabbit pAb (A11369) }

Western blot - IL1β Rabbit pAb (A11369)

Western blot analysis of extracts of various cell lines, using IL1β antibody (A11369) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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