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IL1β Rabbit pAb (A1112)

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ABclonal:IL1β Rabbit pAb
ABclonal:Western blot - IL1β Rabbit pAb (A1112)

Western blot analysis of extracts of various cell lines, using IL1β antibody (A1112) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)

Overview

Product nameIL1β Rabbit pAb
Catalog No.A1112
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1β (NP_000567.1).
SequenceMAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPLDGGIQLRISDHHYSKGFRQAASVVVAMDKLRKMLVPCPQTFQENDLSTFFPFIFEEEPIFFDTWDNEAYVHDAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
Gene ID3553
Swiss protP01584
SynonymsIL-1; IL1-BETA; IL1F2; IL1 beta; IL1B
Calculated MW30kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRAW264.7, Mouse lung, Mouse liver
Cellular locationCytoplasm, Cytoplasmic vesicle, Lysosome, Secreted, autophagosome, cytosol, exosome
Customer validation

(Mouse embryonal fibroblast cell, HUVEC cells, Human corneal epithelial cells, Human cells)

WB(Rattus norvegicus, Mus musculus, Homo sapiens, Escherichia coli, Bos taurus)

IP(Homo sapiens,Mus musculus)

IF(Mus musculus)

IHC(Mus musculus)

ELISA(Mus musculus)

WB(Mus musculus)

IF(Homo sapiens, Mus musculus)

ABclonal:Western blot - IL1β Rabbit pAb (A1112) }

Western blot - IL1β Rabbit pAb (A1112)

Western blot analysis of extracts of various cell lines, using IL1β antibody (A1112) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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