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IL1 beta Rabbit pAb (A1112)

Reviews (5)Publications (34) Datasheet

ABclonal:Western blot - IL1 beta Rabbit pAb (A1112)
ABclonal:Immunofluorescence - IL1 beta Rabbit pAb (A1112)
ABclonal:Immunofluorescence - IL1 beta Rabbit pAb (A1112)
Review
ABclonal: review for IL1 beta Rabbit pAb(A1112)
Review
ABclonal: review for IL1 beta Rabbit pAb(A1112)
Review
ABclonal: review for IL1 beta Rabbit pAb(A1112)
Review
ABclonal: review for IL1 beta Rabbit pAb(A1112)
Review
ABclonal: review for IL1 beta Rabbit pAb(A1112)

Overview

Product nameIL1 beta Rabbit pAb
Catalog No.A1112
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1 beta (NP_000567.1).
SequenceMAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPLDGGIQLRISDHHYSKGFRQAASVVVAMDKLRKMLVPCPQTFQENDLSTFFPFIFEEEPIFFDTWDNEAYVHDAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
Gene ID3553
Swiss protP01584
SynonymsIL-1; IL1-BETA; IL1F2; IL1 beta; IL1B
Calculated MW30kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse liver
Cellular locationCytoplasm, Cytoplasmic vesicle, Lysosome, Secreted, autophagosome, cytosol, exosome
Customer validation

(HUVEC cells, Human corneal epithelial cells, Human cells, Mouse embryonal fibroblast cell)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Escherichia coli, Bos taurus)

ELISA(Mus musculus)

WB(Mus musculus)

IF(Homo sapiens, Mus musculus)

IP(Homo sapiens,Mus musculus)

IF(Mus musculus)

IHC(Mus musculus)

ABclonal:Western blot - IL1 beta Rabbit pAb (A1112) }

Western blot - IL1 beta Rabbit pAb (A1112)

Western blot analysis of extracts of mouse liver, using IL1 beta antibody (A1112) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - IL1 beta Rabbit pAb (A1112) }

Immunofluorescence - IL1 beta Rabbit pAb (A1112)

Immunofluorescence analysis of THP-1 cells using IL1 beta Polyclonal Antibody (A1112) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IL1 beta Rabbit pAb (A1112) }

Immunofluorescence - IL1 beta Rabbit pAb (A1112)

Immunofluorescence analysis of mouse lung using IL1 beta Polyclonal Antibody (A1112) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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