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IL18 Rabbit pAb (A16737)

Publications (12) Datasheet

ABclonal:Western blot - IL18 Rabbit pAb (A16737)

Western blot analysis of extracts of various cell lines, using IL18 antibody (A16737) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - IL18 Rabbit pAb (A16737)

Immunofluorescence analysis of C6 cells using IL18 antibody (A16737) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IL18 Rabbit pAb (A16737)

Immunofluorescence analysis of HeLa cells using IL18 antibody (A16737) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IL18 Rabbit pAb (A16737)

Immunofluorescence analysis of L929 cells using IL18 antibody (A16737) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameIL18 Rabbit pAb
Catalog No.A16737
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant proteinof human IL18.
SequenceEmail for sequence
Gene ID3606
Swiss protQ14116
SynonymsIL18; IGIF; IL-18; IL-1g; IL1F4
Calculated MW21kDa/22kDa
Observed MW22KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesRat brain, Rat spleen
Cellular locationSecreted
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Danio rerio)

IHC(Mus musculus)

ABclonal:Western blot - IL18 Rabbit pAb (A16737) }

Western blot - IL18 Rabbit pAb (A16737)

Western blot analysis of extracts of various cell lines, using IL18 antibody (A16737) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - IL18 Rabbit pAb (A16737) }

Immunofluorescence - IL18 Rabbit pAb (A16737)

Immunofluorescence analysis of C6 cells using IL18 antibody (A16737) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IL18 Rabbit pAb (A16737) }

Immunofluorescence - IL18 Rabbit pAb (A16737)

Immunofluorescence analysis of HeLa cells using IL18 antibody (A16737) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IL18 Rabbit pAb (A16737) }

Immunofluorescence - IL18 Rabbit pAb (A16737)

Immunofluorescence analysis of L929 cells using IL18 antibody (A16737) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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