Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

IL17A Rabbit pAb (A0688)

Publications (31) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human

ABclonal:Western blot - IL17A Rabbit pAb (A0688)

Western blot analysis of various lysates, using IL17A antibody (A0688) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

You may also interested in:

    Overview

    Product nameIL17A Rabbit pAb
    Catalog No.A0688
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene is a member of the IL-17 receptor family which includes five members (IL-17RA-E) and the encoded protein is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. The encoded protein elicits crucial impacts on host defense, cell trafficking, immune modulation, and tissue repair, with a key role in the induction of innate immune defenses. This cytokine stimulates non-hematopoietic cells and promotes chemokine production thereby attracting myeloid cells to inflammatory sites. This cytokine also regulates the activities of NF-kappaB and mitogen-activated protein kinases and can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A plays a pivotal role in various infectious diseases, inflammatory and autoimmune disorders, and cancer. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The lung damage induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL17A.

    Immunogen information

    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1).
    SequenceGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA
    Gene ID3605
    Swiss protQ16552
    SynonymsIL17; CTLA8; IL-17; ILA17; CTLA-8; IL-17A
    Calculated MW18kDa
    Observed MW18-25kDa

    Applications

    ReactivityHuman
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:100 - 1:500
    • IF 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
    Application keyWestern blotting    
    Positive samples
    Cellular locationSecreted
    Customer validation

    WB(Mus musculus, Rattus norvegicus, Homo sapiens)

    IF(Mus musculus, Rattus norvegicus)

    IHC(Mus musculus)

    IHC(Rattus norvegicus, Homo sapiens)

    IF(Rattus norvegicus)

    Cell culture(Mus musculus)

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    IL17A Rabbit pAb images

    ABclonal:Western blot - IL17A Rabbit pAb (A0688)}

    Western blot - IL17A Rabbit pAb (A0688)

    Western blot analysis of various lysates, using IL17A antibody (A0688) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    Inquire About This Product

    Submit your question about A0688 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Related Targets

    Based on existing publications, the following genes are closely related to research on IL17A. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to IL17A. (Distance between topics and target gene indicate popularity.) IL17A

    * Data provided by citexs.com, for reference only.

    Publishing research using A0688? Please let us know so that we can cite the reference in this datasheet.

    Antibodies (2)

    ELISA Kits (1)

    Proteins (4)

    Secondary Antibodies (22)

    * For research use only. Not for therapeutic or diagnostic purposes.

    Select size and quantity
    Contact us to order