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IL17A Rabbit pAb (A0688)

Publications (12) Datasheet

ABclonal:Western blot - IL17A Rabbit pAb (A0688)
ABclonal:Immunofluorescence - IL17A Rabbit pAb (A0688)

Overview

Product nameIL17A Rabbit pAb
Catalog No.A0688
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1).
SequenceGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA
Gene ID3605
Swiss protQ16552
SynonymsIL17A; CTLA-8; CTLA8; IL-17; IL-17A; IL17
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesTHP-1
Cellular locationSecreted
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Mus musculus)

IHC(Mus musculus)

ABclonal:Western blot - IL17A Rabbit pAb (A0688) }

Western blot - IL17A Rabbit pAb (A0688)

Western blot analysis of extracts of THP-1 cells, using IL17A antibody (A0688) at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37℃ for overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - IL17A Rabbit pAb (A0688) }

Immunofluorescence - IL17A Rabbit pAb (A0688)

Immunofluorescence analysis of L929 cells using IL17A Rabbit pAb (A0688) at dilution of 1:100. Blue: DAPI for nuclear staining.

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