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IL17A Polyclonal Antibody (A0688)

Publications (8) Datasheet

ABclonal:Western blot - IL17A antibody (A0688)

Overview

Product nameIL17A Polyclonal Antibody
Catalog No.A0688
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1).
SequenceGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA
Gene ID3605
Swiss protQ16552
SynonymsCTLA-8; CTLA8; IL-17; IL-17A; IL17
Calculated MW17kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:200 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-937, COS7, Jurkat, THP-1, Mouse liver, Mouse heart, Mouse thymus, Rat thymus, Rat liver
Cellular locationSecreted
Customer validation

WB(Mouse, Rat, Human)

IF(Mouse)

IHC(Mouse)

ABclonal:Western blot - IL17A antibody (A0688)}

Western blot - IL17A antibody (A0688)

Western blot analysis of extracts of various cell lines, using IL17A antibody (A0688) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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