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IL13 Rabbit pAb (A2089)

Publications (2) Datasheet

ABclonal:Western blot - IL13 Polyclonal Antibody (A2089)

Western blot analysis of extracts of mouse pancreas, using IL13 antibody (A2089) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 2min.

ABclonal:Immunohistochemistry - IL13 Polyclonal Antibody (A2089)

Immunohistochemistry of paraffin-embedded mouse brain using IL13 antibody (A2089) (40x lens).

ABclonal:Immunohistochemistry - IL13 Polyclonal Antibody (A2089)

Immunohistochemistry of paraffin-embedded rat brain using IL13 antibody (A2089) (40x lens).

ABclonal:Immunohistochemistry - IL13 Polyclonal Antibody (A2089)

Immunohistochemistry of paraffin-embedded human stomach using IL13 antibody (A2089) (40x lens).

Overview

Product nameIL13 Rabbit pAb
Catalog No.A2089
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-146 of human IL13 (NP_002179.2).
SequenceLTCLGGFASPGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGQFN
Gene ID3596
Swiss protP35225
SynonymsIL13; IL-13; P600
Calculated MW15kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse pancreas
Cellular locationSecreted
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - IL13 Polyclonal Antibody (A2089) }

Western blot - IL13 Polyclonal Antibody (A2089)

Western blot analysis of extracts of mouse pancreas, using IL13 antibody (A2089) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 2min.
ABclonal:Immunohistochemistry - IL13 Polyclonal Antibody (A2089) }

Immunohistochemistry - IL13 Polyclonal Antibody (A2089)

Immunohistochemistry of paraffin-embedded mouse brain using IL13 antibody (A2089) (40x lens).
ABclonal:Immunohistochemistry - IL13 Polyclonal Antibody (A2089) }

Immunohistochemistry - IL13 Polyclonal Antibody (A2089)

Immunohistochemistry of paraffin-embedded rat brain using IL13 antibody (A2089) (40x lens).
ABclonal:Immunohistochemistry - IL13 Polyclonal Antibody (A2089) }

Immunohistochemistry - IL13 Polyclonal Antibody (A2089)

Immunohistochemistry of paraffin-embedded human stomach using IL13 antibody (A2089) (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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