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IKKα Rabbit pAb (A2062)

Reviews (4)Publications (5) Datasheet

ABclonal:Western blot - IKKα Rabbit pAb (A2062)
ABclonal:Immunohistochemistry - IKKα Rabbit pAb (A2062)
ABclonal:Immunohistochemistry - IKKα Rabbit pAb (A2062)
ABclonal:Immunohistochemistry - IKKα Rabbit pAb (A2062)
ABclonal:Immunofluorescence - IKKα Rabbit pAb (A2062)
ABclonal:Immunofluorescence - IKKα Rabbit pAb (A2062)
ABclonal:Immunoprecipitation - IKKα Rabbit pAb (A2062)
Review
ABclonal: review for IKKα Rabbit pAb(A2062)
Review
ABclonal: review for IKKα Rabbit pAb(A2062)
Review
ABclonal: review for IKKα Rabbit pAb(A2062)
Review
ABclonal: review for IKKα Rabbit pAb(A2062)

Overview

Product nameIKKα Rabbit pAb
Catalog No.A2062
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 650 to the C-terminus of human IKKα (NP_001269.3).
SequenceIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE
Gene ID1147
Swiss protO15111
SynonymsCHUK; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16; IKK alpha
Calculated MW84kDa
Observed MW94kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, HT-29
Cellular locationCytoplasm, Nucleus
Customer validation

(H460, Raw264.7, Rat H9c2 cells)

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

ABclonal:Western blot - IKKα Rabbit pAb (A2062) }

Western blot - IKKα Rabbit pAb (A2062)

Western blot analysis of extracts of various cell lines, using IKKα antibody (A2062) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - IKKα Rabbit pAb (A2062) }

Immunohistochemistry - IKKα Rabbit pAb (A2062)

Immunohistochemistry of paraffin-embedded rat liver using CHUK antibody (A2062) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - IKKα Rabbit pAb (A2062) }

Immunohistochemistry - IKKα Rabbit pAb (A2062)

Immunohistochemistry of paraffin-embedded human tonsil using CHUK antibody (A2062) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - IKKα Rabbit pAb (A2062) }

Immunohistochemistry - IKKα Rabbit pAb (A2062)

Immunohistochemistry of paraffin-embedded mouse kidney using CHUK antibody (A2062) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - IKKα Rabbit pAb (A2062) }

Immunofluorescence - IKKα Rabbit pAb (A2062)

Immunofluorescence analysis of NIH/3T3 cells using CHUK antibody (A2062) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IKKα Rabbit pAb (A2062) }

Immunofluorescence - IKKα Rabbit pAb (A2062)

Immunofluorescence analysis of U-2 OS cells using CHUK antibody (A2062) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - IKKα Rabbit pAb (A2062) }

Immunoprecipitation - IKKα Rabbit pAb (A2062)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug IKKα antibody (A2062). Western blot was performed from the immunoprecipitate using IKKα antibody (A2062) at a dilition of 1:1000.

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