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IKK alpha Polyclonal Antibody (A2062)

Reviews (4)Publications (4) Datasheet

ABclonal:Western blot - IKK alpha Polyclonal Antibody (A2062)
ABclonal:Immunohistochemistry - IKK alpha Polyclonal Antibody (A2062)
ABclonal:Immunohistochemistry - IKK alpha Polyclonal Antibody (A2062)
ABclonal:Immunofluorescence - IKK alpha Polyclonal Antibody (A2062)
ABclonal:Immunofluorescence - IKK alpha Polyclonal Antibody (A2062)
ABclonal:Immunoprecipitation - IKK alpha Polyclonal Antibody (A2062)
Review
ABclonal: review for IKK alpha Polyclonal Antibody(A2062)
Review
ABclonal: review for IKK alpha Polyclonal Antibody(A2062)
Review
ABclonal: review for IKK alpha Polyclonal Antibody(A2062)
Review
ABclonal: review for IKK alpha Polyclonal Antibody(A2062)

Overview

Product nameIKK alpha Polyclonal Antibody
Catalog No.A2062
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 650 to the C-terminus of human IKK alpha (NP_001269.3).
SequenceIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE
Gene ID1147
Swiss protO15111
SynonymsCHUK; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16; conserved helix-loop-helix ubiquitous kinase
Calculated MW84kDa
Observed MW94kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, HT-29
Cellular locationCytoplasm, Nucleus
Customer validation

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WB(Human, Mouse, Human, Mouse)

ABclonal:Western blot - IKK alpha Polyclonal Antibody (A2062) }

Western blot - IKK alpha Polyclonal Antibody (A2062)

Western blot analysis of extracts of various cell lines, using IKK alpha antibody (A2062) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - IKK alpha Polyclonal Antibody (A2062) }

Immunohistochemistry - IKK alpha Polyclonal Antibody (A2062)

Immunohistochemistry of paraffin-embedded rat brain using IKK alpha antibody (A2062) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - IKK alpha Polyclonal Antibody (A2062) }

Immunohistochemistry - IKK alpha Polyclonal Antibody (A2062)

Immunohistochemistry of paraffin-embedded mouse kidney using IKK alpha antibody (A2062) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - IKK alpha Polyclonal Antibody (A2062) }

Immunofluorescence - IKK alpha Polyclonal Antibody (A2062)

Immunofluorescence analysis of NIH/3T3 cells using CHUK antibody (A2062) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IKK alpha Polyclonal Antibody (A2062) }

Immunofluorescence - IKK alpha Polyclonal Antibody (A2062)

Immunofluorescence analysis of U-2 OS cells using CHUK antibody (A2062) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - IKK alpha Polyclonal Antibody (A2062) }

Immunoprecipitation - IKK alpha Polyclonal Antibody (A2062)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug IKK alpha antibody (A2062). Western blot was performed from the immunoprecipitate using IKK alpha antibody (A2062) at a dilition of 1:1000.

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