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IKB alpha Polyclonal Antibody (A1187)

Reviews (6)Publications (8) Datasheet

ABclonal:Western blot - IKB alpha Polyclonal Antibody (A1187)
Review
ABclonal: review for IKB alpha Polyclonal Antibody(A1187)
Review
ABclonal: review for IKB alpha Polyclonal Antibody(A1187)
Review
ABclonal: review for IKB alpha Polyclonal Antibody(A1187)
Review
ABclonal: review for IKB alpha Polyclonal Antibody(A1187)
Review
ABclonal: review for IKB alpha Polyclonal Antibody(A1187)
Review
ABclonal: review for IKB alpha Polyclonal Antibody(A1187)

Overview

Product nameIKB alpha Polyclonal Antibody
Catalog No.A1187
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IKB alpha (NP_065390.1).
SequenceMFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Gene ID4792
Swiss protP25963
SynonymsIKBA; MAD-3; NFKBI; IKB alpha; NFKBIA; EDAID2
Calculated MW35kDa
Observed MW39KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesDU145, MCF7, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

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WB(Human, Mouse)

IF(Human)

IF(Mouse)

ABclonal:Western blot - IKB alpha Polyclonal Antibody (A1187) }

Western blot - IKB alpha Polyclonal Antibody (A1187)

Western blot analysis of extracts of various cell lines, using IKB alpha antibody (A1187) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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