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IκBα Rabbit pAb (A1187)

Reviews (6)Publications (13) Datasheet

ABclonal:Western blot - IκBα Rabbit pAb (A1187)
Review
ABclonal: review for IκBα Rabbit pAb(A1187)
Review
ABclonal: review for IκBα Rabbit pAb(A1187)
Review
ABclonal: review for IκBα Rabbit pAb(A1187)
Review
ABclonal: review for IκBα Rabbit pAb(A1187)
Review
ABclonal: review for IκBα Rabbit pAb(A1187)
Review
ABclonal: review for IκBα Rabbit pAb(A1187)

Overview

Product nameIκBα Rabbit pAb
Catalog No.A1187
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IκBα (NP_065390.1).
SequenceMFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Gene ID4792
Swiss protP25963
SynonymsIKBA; MAD-3; NFKBI; IKB alpha; NFKBIA; EDAID2
Calculated MW35kDa
Observed MW39KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesDU145, MCF7, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse splenocytes, HepG2, Human glioma, Hela, Mouse embryonal fibroblast cell)

WB(Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus, Schisandra chinensis)

IF(Homo sapiens)

ABclonal:Western blot - IκBα Rabbit pAb (A1187) }

Western blot - IκBα Rabbit pAb (A1187)

Western blot analysis of extracts of various cell lines, using IκBα antibody (A1187) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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