Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Cation-independent M6PR (IGF2R) Rabbit mAb (A10916)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Cation-independent M6PR (IGF2R) Rabbit mAb (A10916)

Western blot analysis of extracts of various cell lines, using Cation-independent M6PR (Cation-independent M6PR (IGF2R)) antibody (A10916).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

You may also interested in:

Overview

Product name Cation-independent M6PR (IGF2R) Rabbit mAb
Catalog No. A10916
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).

Immunogen information

Immunogen Recombinant protein of human Cation-independent M6PR (Cation-independent M6PR (IGF2R))
Sequence Email for sequence
Gene ID 3482
Swiss prot P11717
Synonyms MPR1; MPRI; CD222; CIMPR; M6P-R; MPR300; CI-M6PR; MPR 300; M6P/IGF2R; Cation-independent M6PR (IGF2R)
Calculated MW 274kDa
Observed MW 274kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HepG2, SW480
Cellular location Lysosome membrane, Single-pass type I membrane protein

Research Area

Cation-independent M6PR (IGF2R) Rabbit mAb images

ABclonal:Western blot - Cation-independent M6PR (IGF2R) Rabbit mAb (A10916)}

Western blot - Cation-independent M6PR (IGF2R) Rabbit mAb (A10916)

Western blot analysis of extracts of various cell lines, using Cation-independent M6PR (Cation-independent M6PR (IGF2R)) antibody (A10916).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Inquire About This Product

Submit your question about A10916 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on IGF2R. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IGF2R. (Distance between topics and target gene indicate popularity.) IGF2R

* Data provided by citexs.com, for reference only.

Publishing research using A10916? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

!OUT OF STOCK

See Below for Alternatives
Contact us to order