Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

IFN gamma Rabbit pAb (A12450)

Publications (20) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - IFN gamma Rabbit pAb (A12450)

Western blot analysis of Recombinant Human IFN-gamma Protein, using IFN gamma antibody (A12450) at 1:9000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - IFN gamma Rabbit pAb (A12450)

Immunohistochemistry analysis of paraffin-embedded human lung using IFN gamma antibody (A12450) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name IFN gamma Rabbit pAb
Catalog No. A12450
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 24-161 of IFN gamma (NP_997468.1).
Sequence VFGISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEE
Gene ID 3458
Swiss prot P01579
Synonyms IFG; IFI; IMD69; IFN gamma
Calculated MW 19kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Recombinant Human IFN-gamma Protein
Cellular location Secreted
Customer validation

WB (Mus musculus, Rattus norvegicus, Homo sapiens)

IHC (Mus musculus, Homo sapiens)

ELISA (Mus musculus)

IF (Mus musculus)

IP (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

IFN gamma Rabbit pAb images

ABclonal:Western blot - IFN gamma Rabbit pAb (A12450)}

Western blot - IFN gamma Rabbit pAb (A12450)

Western blot analysis of Recombinant Human IFN-gamma Protein, using IFN gamma antibody (A12450) at 1:9000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - IFN gamma Rabbit pAb (A12450)}

Immunohistochemistry - IFN gamma Rabbit pAb (A12450)

Immunohistochemistry analysis of paraffin-embedded human lung using IFN gamma antibody (A12450) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on IFNG. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IFNG. (Distance between topics and target gene indicate popularity.) IFNG

* Data provided by citexs.com, for reference only.

Publishing research using A12450? Please let us know so that we can cite the reference in this datasheet.

Proteins (2)

Antibodies (5)

ELISA Kits (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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