Products > Catalog Antibodies > Polyclonal Antibodies

IFNG Rabbit pAb (A12450)

Publications (4) Datasheet

ABclonal:Western blot - IFNG Polyclonal Antibody (A12450)

Western blot analysis of extracts of various cell lines, using IFNG antibody (A12450) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - IFNG Polyclonal Antibody (A12450)

Immunohistochemistry of paraffin-embedded human lung using IFNG antibody (A12450) at dilution of 1:100 (40x lens).

Overview

Product nameIFNG Rabbit pAb
Catalog No.A12450
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFNG (NP_000610.2).
SequenceQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ
Gene ID3458
Swiss protP01579
SynonymsIFNG; IFG; IFI
Calculated MW19kDa
Observed MW17kDa/23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHT-29, PC-3, U-251MG, Mouse brain, Rat brain
Cellular locationSecreted
Customer validation

WB(Mus musculus, Rattus norvegicus)

ABclonal:Western blot - IFNG Polyclonal Antibody (A12450) }

Western blot - IFNG Polyclonal Antibody (A12450)

Western blot analysis of extracts of various cell lines, using IFNG antibody (A12450) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - IFNG Polyclonal Antibody (A12450) }

Immunohistochemistry - IFNG Polyclonal Antibody (A12450)

Immunohistochemistry of paraffin-embedded human lung using IFNG antibody (A12450) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A12450 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A12450? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order