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IFIH1 Rabbit pAb (A13645)

Publications (2) Datasheet

ABclonal:Western blot - IFIH1 Rabbit pAb (A13645)

Western blot analysis of extracts of Mouse liver, using IFIH1 antibody (A13645) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded human esophageal cancer using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded human placenta using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded mouse lung using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded mouse spleen using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded rat lung using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).

ABclonal:Immunofluorescence - IFIH1 Rabbit pAb (A13645)

Immunofluorescence analysis of THP-1 cells using IFIH1 Rabbit pAb (A13645) at dilution of 1:100 (40x lens).THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 2 hours (top left). Blue: DAPI for nuclear staining.

Overview

Product nameIFIH1 Rabbit pAb
Catalog No.A13645
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human IFIH1 (NP_071451.2).
SequenceMSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSES
Gene ID64135
Swiss protQ9BYX4
SynonymsIFIH1; AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2; SGMRT1
Calculated MW25kDa/116kDa
Observed MW140KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Cynoglossus semilaevis, Gallus gallus)

ABclonal:Western blot - IFIH1 Rabbit pAb (A13645) }

Western blot - IFIH1 Rabbit pAb (A13645)

Western blot analysis of extracts of Mouse liver, using IFIH1 antibody (A13645) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645) }

Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded human esophageal cancer using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645) }

Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded human placenta using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645) }

Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded mouse lung using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645) }

Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded mouse spleen using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - IFIH1 Rabbit pAb (A13645) }

Immunohistochemistry - IFIH1 Rabbit pAb (A13645)

Immunohistochemistry of paraffin-embedded rat lung using IFIH1 Rabbit pAb (A13645) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - IFIH1 Rabbit pAb (A13645) }

Immunofluorescence - IFIH1 Rabbit pAb (A13645)

Immunofluorescence analysis of THP-1 cells using IFIH1 Rabbit pAb (A13645) at dilution of 1:100 (40x lens).THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 2 hours (top left). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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