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ICAM-1/CD54 Rabbit pAb (A5597)

Publications (10) Datasheet

ABclonal:Western blot - ICAM-1/CD54 Rabbit pAb (A5597)

Western blot analysis of extracts of A-549 cells, using ICAM-1/CD54 antibody (A5597) at 1:1000 dilution.A-549 cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.A-549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597)

Immunohistochemistry of paraffin-embedded human gastric cancer using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597)

Immunohistochemistry of paraffin-embedded human lung cancer using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597)

Immunohistochemistry of paraffin-embedded human tonsil using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - ICAM-1/CD54 Rabbit pAb (A5597)

Immunofluorescence analysis of L929 cells using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameICAM-1/CD54 Rabbit pAb
Catalog No.A5597
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 250-480 of human ICAM-1/CD54 (NP_000192.2).
SequenceVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYE
Gene ID3383
Swiss protP05362
SynonymsBB2; CD54; P3.58; ICAM1
Calculated MW57kDa
Observed MW89KDa/92KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

WB(Homo sapiens,Mus musculus, Rattus norvegicus, Sus scrofa)

IF(Sus scrofa)

ABclonal:Western blot - ICAM-1/CD54 Rabbit pAb (A5597) }

Western blot - ICAM-1/CD54 Rabbit pAb (A5597)

Western blot analysis of extracts of A-549 cells, using ICAM-1/CD54 antibody (A5597) at 1:1000 dilution.A-549 cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.A-549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597) }

Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597)

Immunohistochemistry of paraffin-embedded human gastric cancer using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597) }

Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597)

Immunohistochemistry of paraffin-embedded human lung cancer using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597) }

Immunohistochemistry - ICAM-1/CD54 Rabbit pAb (A5597)

Immunohistochemistry of paraffin-embedded human tonsil using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - ICAM-1/CD54 Rabbit pAb (A5597) }

Immunofluorescence - ICAM-1/CD54 Rabbit pAb (A5597)

Immunofluorescence analysis of L929 cells using ICAM-1/CD54 Rabbit pAb (A5597) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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