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Human IgG Rabbit mAb (A19711)

Publications (5) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human

ABclonal:Western blot - Human IgG Rabbit mAb (A19711)

Western blot analysis of extracts of Human plasma, using Human IgG antibody (A19711) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Human IgG Rabbit mAb (A19711)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using Human IgG antibody (A19711) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Human IgG Rabbit mAb (A19711)

Immunohistochemistry of paraffin-embedded Human tonsil using Human IgG antibody (A19711) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameHuman IgG Rabbit mAb
Catalog No.A19711
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.
ImmunogenHuman IgG
SequenceEmail for sequence
Gene ID3500
Swiss protP01857, P01859, P01860, P01861
Synonyms
Calculated MW52kDa
Observed MW50KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:300
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive sampleshuman plasma
Cellular location
Customer validation

WB(Homo sapiens)

Co-IP(Homo sapiens)

    ABclonal:Western blot - Human IgG Rabbit mAb (A19711)}

    Western blot - Human IgG Rabbit mAb (A19711)

    Western blot analysis of extracts of Human plasma, using Human IgG antibody (A19711) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - Human IgG Rabbit mAb (A19711)}

    Immunohistochemistry - Human IgG Rabbit mAb (A19711)

    Immunohistochemistry of paraffin-embedded Human colon carcinoma using Human IgG antibody (A19711) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - Human IgG Rabbit mAb (A19711)}

    Immunohistochemistry - Human IgG Rabbit mAb (A19711)

    Immunohistochemistry of paraffin-embedded Human tonsil using Human IgG antibody (A19711) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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