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Histone H3 Rabbit mAb (A17562)

Datasheet

ABclonal:Western blot - Histone H3 Rabbit mAb (A17562)
ABclonal:Immunohistochemistry - Histone H3 Rabbit mAb (A17562)
ABclonal:Immunohistochemistry - Histone H3 Rabbit mAb (A17562)
ABclonal:Immunohistochemistry - Histone H3 Rabbit mAb (A17562)

Overview

Product nameHistone H3 Rabbit mAb
Catalog No.A17562
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
ImmunogenA synthesized peptide derived from human Histone H3
SequenceEmail for sequence
Gene ID8350
Swiss protP68431
SynonymsH3/A; H3FA
Calculated MW15kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HepG2, NIH/3T3, Raji, C6, Mouse spleen, Rat lung
Cellular location
ABclonal:Western blot - Histone H3 Rabbit mAb (A17562) }

Western blot - Histone H3 Rabbit mAb (A17562)

Western blot analysis of extracts of various cell lines, using Histone H3 Rabbit mAb (A17562) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Histone H3 Rabbit mAb (A17562) }

Immunohistochemistry - Histone H3 Rabbit mAb (A17562)

Immunohistochemistry of paraffin-embedded human colon using Histone H3 Rabbit mAb (A17562) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Histone H3 Rabbit mAb (A17562) }

Immunohistochemistry - Histone H3 Rabbit mAb (A17562)

Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 Rabbit mAb (A17562) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Histone H3 Rabbit mAb (A17562) }

Immunohistochemistry - Histone H3 Rabbit mAb (A17562)

Immunohistochemistry of paraffin-embedded rat spleen using Histone H3 Rabbit mAb (A17562) at dilution of 1:100 (40x lens).

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