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Heme Oxygenase 1 Rabbit mAb (A19062)

Datasheet

ABclonal:Heme Oxygenase 1 Rabbit mAb
ABclonal:Western blot - Heme Oxygenase 1 Rabbit mAb (A19062)
ABclonal:Immunohistochemistry - Heme Oxygenase 1 Rabbit mAb (A19062)
ABclonal:Immunohistochemistry - Heme Oxygenase 1 Rabbit mAb (A19062)

Overview

Product nameHeme Oxygenase 1 Rabbit mAb
Catalog No.A19062
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human Heme Oxygenase 1
SequenceEmail for sequence
Gene ID3162
Swiss protP09601
SynonymsHMOX1; HMOX1D; HO-1; HSP32; bK286B10
Calculated MW33kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse spleen, Mouse kidney, Rat kidney
Cellular location
ABclonal:Western blot - Heme Oxygenase 1 Rabbit mAb (A19062) }

Western blot - Heme Oxygenase 1 Rabbit mAb (A19062)

Western blot analysis of extracts of various cell lines, using Heme Oxygenase 1 antibody (A19062) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Heme Oxygenase 1 Rabbit mAb (A19062) }

Immunohistochemistry - Heme Oxygenase 1 Rabbit mAb (A19062)

Immunohistochemistry of paraffin-embedded human kidney cancer using Heme Oxygenase 1 Rabbit mAb (A19062) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Heme Oxygenase 1 Rabbit mAb (A19062) }

Immunohistochemistry - Heme Oxygenase 1 Rabbit mAb (A19062)

Immunohistochemistry of paraffin-embedded mouse liver using Heme Oxygenase 1 Rabbit mAb (A19062) at dilution of 1:100 (40x lens).

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