Products > Catalog Antibodies > Polyclonal Antibodies

Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Review (1)Publications (26) Datasheet

ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of extracts of various cell lines, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (A1346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunohistochemistry of paraffin-embedded human liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunofluorescence analysis of HeLa cells using Heme Oxygenase 1 (HO-1/HMOX1) Polyclonal Antibody (A1346) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb(A1346)

Overview

Product nameHeme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb
Catalog No.A1346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human Heme Oxygenase 1 (HO-1/HMOX1) (NP_002124.1).
SequenceMERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM
Gene ID3162
Swiss protP09601
SynonymsHMOX1; HMOX1D; HO-1; HSP32; bK286B10
Calculated MW32kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen, Mouse kidney, Rat kidney
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein
Customer validation

(Mouse liver)

WB(Mus musculus, Homo sapiens, Cyprinus carpio, Rattus norvegicus, Struthio camelus)

ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) }

Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of extracts of various cell lines, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (A1346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) }

Immunohistochemistry - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunohistochemistry of paraffin-embedded human liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) }

Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunofluorescence analysis of HeLa cells using Heme Oxygenase 1 (HO-1/HMOX1) Polyclonal Antibody (A1346) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A1346

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A1346 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A1346? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order