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HSPA1A Polyclonal Antibody (A0284)

Reviews (2)Publications (5) Datasheet

ABclonal:Western blot - HSPA1A antibody (A0284)
ABclonal:Immunofluorescence - HSPA1A antibody (A0284)

Overview

Product nameHSPA1A Polyclonal Antibody
Catalog No.A0284
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
ImmunogenA synthetic peptide of human HSPA1A
SequenceEmail for sequence
Gene ID3303
Swiss protP0DMV8
SynonymsHEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1
Calculated MW70kDa
Observed MW72kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, COS1, COS7, U2OS, 293T, HepG2
Cellular location
Customer validation

IF(HK2)

WB(HeLa, Human, Rat, Mouse)

IF(Human)

ABclonal:Western blot - HSPA1A antibody (A0284)}

Western blot - HSPA1A antibody (A0284)

Western blot analysis of extracts of various cell lines, using HSPA1A antibody (A0284) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - HSPA1A antibody (A0284)}

Immunofluorescence - HSPA1A antibody (A0284)

Immunofluorescence analysis of U2OS cells using HSPA1A antibody (A0284). Blue: DAPI for nuclear staining.

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