Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] Hsp90β Rabbit pAb (A1087)

KO/KDValidated

Reviews (10) Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] Hsp90β Rabbit pAb (A1087)

Western blot analysis of lysates from wild type (WT) and Hsp90β knockout (KO) HeLa cells, using [KO Validated] Hsp90β Rabbit pAb (A1087) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Rat brain tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Mouse kidney tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Rat testis tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Human small intestine tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)
Review
ABclonal: review for [KO Validated] Hsp90β Rabbit pAb(A1087)

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Overview

Product name [KO Validated] Hsp90β Rabbit pAb
Catalog No. A1087
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2).
Sequence MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEE
Gene ID 3326
Swiss prot P08238
Synonyms HSP84; HSPC2; HSPCB; D6S182; HSP90B; 0β
Calculated MW 83kDa
Observed MW 80kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples K-562, DU145, SW620, COS-7, NIH/3T3, HeLa
Cellular location Cytoplasm, Melanosome
Customer validation

IF (Human liver cell)

WB (Human liver cell, Rat cellsRattus norvegicus, Rattus norvegicus, Gallus gallus, Mus musculus, Homo sapiens)

IHC (Mouse Embryonic Stem Cells)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Hsp90β Rabbit pAb images

ABclonal:Western blot - [KO Validated] Hsp90β Rabbit pAb (A1087)}

Western blot - [KO Validated] Hsp90β Rabbit pAb (A1087)

Western blot analysis of lysates from wild type (WT) and Hsp90β knockout (KO) HeLa cells, using [KO Validated] Hsp90β Rabbit pAb (A1087) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)}

Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Rat brain tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)}

Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Mouse kidney tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)}

Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Rat testis tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)}

Immunohistochemistry - [KO Validated] Hsp90β Rabbit pAb (A1087)

Immunohistochemistry analysis of Hsp90β in paraffin-embedded Human small intestine tissue using [KO Validated] Hsp90β Rabbit pAb (A1087) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on HSP90AB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HSP90AB1. (Distance between topics and target gene indicate popularity.) HSP90AB1

* Data provided by citexs.com, for reference only.

Publishing research using A1087? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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