Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

HSP70 Rabbit pAb (A0284)

Reviews (2) Publications (25) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - HSP70 Rabbit pAb (A0284)

Western blot analysis of various lysates using HSP70 Rabbit pAb (A0284) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.

ABclonal:Western blot - HSP70 Rabbit pAb (A0284)

Western blot analysis of lysates from Mouse brain using HSP70 Rabbit pAb(A0284) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:60s.

ABclonal:Immunohistochemistry - HSP70 Rabbit pAb (A0284)

Immunohistochemistry analysis of HSP70 in paraffin-embedded rat kidney using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HSP70 Rabbit pAb (A0284)

Immunohistochemistry analysis of HSP70 in paraffin-embedded mouse spinal cord using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of A549 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of U-2 OS cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of NIH/3T3 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of PC-12 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of HeLa cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for HSP70 Rabbit pAb(A0284)
Review
ABclonal: review for HSP70 Rabbit pAb(A0284)

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Overview

Product name HSP70 Rabbit pAb
Catalog No. A0284
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3).
Sequence KITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE
Gene ID 3303
Swiss prot P0DMV8
Synonyms HSP70; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Calculated MW 70kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse brain, 293T, HeLa, A549
Cellular location blood microparticle, centriole, centrosome, cytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, extracellular region, focal adhesion, mitochondrion, nuclear speck, nucleoplasm, nucleus, perinuclear region of cytoplasm, plasma membrane
Customer validation

IF (HK2Homo sapiens, Homo sapiens, Mus musculus)

WB (HeLaRattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus)

IHC (Mus musculus)

qPCR (Homo sapiens)

Co-IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

HSP70 Rabbit pAb images

ABclonal:Western blot - HSP70 Rabbit pAb (A0284)}

Western blot - HSP70 Rabbit pAb (A0284)

Western blot analysis of various lysates using HSP70 Rabbit pAb (A0284) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.
ABclonal:Western blot - HSP70 Rabbit pAb (A0284)}

Western blot - HSP70 Rabbit pAb (A0284)

Western blot analysis of lysates from Mouse brain using HSP70 Rabbit pAb(A0284) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:60s.
ABclonal:Immunohistochemistry - HSP70 Rabbit pAb (A0284)}

Immunohistochemistry - HSP70 Rabbit pAb (A0284)

Immunohistochemistry analysis of HSP70 in paraffin-embedded rat kidney using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HSP70 Rabbit pAb (A0284)}

Immunohistochemistry - HSP70 Rabbit pAb (A0284)

Immunohistochemistry analysis of HSP70 in paraffin-embedded mouse spinal cord using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)}

Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of A549 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)}

Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of U-2 OS cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)}

Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of NIH/3T3 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)}

Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of PC-12 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)}

Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of HeLa cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on HSPA1A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HSPA1A. (Distance between topics and target gene indicate popularity.) HSPA1A

* Data provided by citexs.com, for reference only.

Publishing research using A0284? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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