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HSP70 Rabbit pAb (A0284)

Reviews (2)Publications (10) Datasheet

ABclonal:Western blot - HSP70 Rabbit pAb (A0284)
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284)
Review
ABclonal: review for HSP70 Rabbit pAb(A0284)
Review
ABclonal: review for HSP70 Rabbit pAb(A0284)

Overview

Product nameHSP70 Rabbit pAb
Catalog No.A0284
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 540 to the C-terminus of human HSP70 (NP_005336.3).
SequenceNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD
Gene ID3303
Swiss protP0DMV8
SynonymsHSPA1A; HEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1; HSP70
Calculated MW70kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, COS-1, COS-7, U2OS, 293T, HepG2
Cellular location
Customer validation

(HK2, HeLa)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IF(Homo sapiens)

Co-IP(Homo sapiens)

ABclonal:Western blot - HSP70 Rabbit pAb (A0284) }

Western blot - HSP70 Rabbit pAb (A0284)

Western blot analysis of extracts of various cell lines, using HSP70 antibody (A0284) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284) }

Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of U2OS cells using HSP70 antibody (A0284). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HSP70 Rabbit pAb (A0284) }

Immunofluorescence - HSP70 Rabbit pAb (A0284)

Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody (A0284) at dilution of 1:100. Blue: DAPI for nuclear staining.

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