Products > Catalog Antibodies > Polyclonal Antibodies

HRas Rabbit pAb (A7901)

Datasheet

ABclonal:Western blot - HRas Polyclonal Antibody (A7901)
ABclonal:Immunohistochemistry - HRas Polyclonal Antibody (A7901)
ABclonal:Immunohistochemistry - HRas Polyclonal Antibody (A7901)

Overview

Product nameHRas Rabbit pAb
Catalog No.A7901
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
ImmunogenA synthetic peptide of human HRAS
SequenceEmail for sequence
Gene ID3265
Swiss protP01112
SynonymsC-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; RASH1; p21ras; GTPase HRAS; HRAS
Calculated MW18kDa/21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSKOV3, Mouse brain, Mouse skeletal muscle, Mouse kidney, Rat brain
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Golgi apparatus, Golgi apparatus membrane, Lipid-anchor, Lipid-anchor, Nucleus, perinuclear region
ABclonal:Western blot - HRas Polyclonal Antibody (A7901) }

Western blot - HRas Polyclonal Antibody (A7901)

Western blot analysis of extracts of various cell lines, using HRAS antibody (A7901) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.
ABclonal:Immunohistochemistry - HRas Polyclonal Antibody (A7901) }

Immunohistochemistry - HRas Polyclonal Antibody (A7901)

Immunohistochemistry of paraffin-embedded human gastric using HRAS antibody (A7901) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - HRas Polyclonal Antibody (A7901) }

Immunohistochemistry - HRas Polyclonal Antibody (A7901)

Immunohistochemistry of paraffin-embedded human gastric cancer using HRAS antibody (A7901) at dilution of 1:100 (40x lens).

Inquire About This Product

Submit your question about A7901 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order