Product Type > Antibodies > Secondary Antibodies

HRP-conjugated Mouse Anti-Rabbit IgG (H+L) (AS079)

Publication (1) Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Western blot - HRP-conjugated  Mouse Anti-Rabbit IgG (H+L) (AS079)

Western blot analysis of lysates from HeLa cells, using β-Actin (AC026) antibody as the primary antibody at dilution of 1:10000.
Secondary antibody: using HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L) antibody (AS079)) at 1:5000-1:10000 dilution.

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Overview

Product name HRP-conjugated Mouse Anti-Rabbit IgG (H+L)
Catalog No. AS079
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Rabbit IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW 36kDa

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:5000 - 1:20000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location
Customer validation

WB (Mus musculus)

HRP-conjugated Mouse Anti-Rabbit IgG (H+L) images

ABclonal:Western blot - HRP-conjugated  Mouse Anti-Rabbit IgG (H+L) (AS079)}

Western blot - HRP-conjugated Mouse Anti-Rabbit IgG (H+L) (AS079)

Western blot analysis of lysates from HeLa cells, using β-Actin (AC026) antibody as the primary antibody at dilution of 1:10000.
Secondary antibody: using HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L) antibody (AS079)) at 1:5000-1:10000 dilution.

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Related Targets

Based on existing publications, the following genes are closely related to research on Mouse Anti-Rabbit. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Mouse Anti-Rabbit. (Distance between topics and target gene indicate popularity.) Mouse Anti-Rabbit

* Data provided by citexs.com, for reference only.

Publishing research using AS079? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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