Product Type > Antibodies > Secondary Antibodies

HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)

Publications (38) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Western blot - HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)

Western blot analysis of extracts of Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain antibody (AS063) at 1:5000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain
Catalog No. AS063
Host species Goat
Purification method Affinity purification
Isotype IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Rabbit IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW 55kDa

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000-10000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Rabbit IgG, Mouse IgG
Cellular location
Customer validation

WB (Sus scrofa, Mus musculus, Homo sapiens, Rattus norvegicus, Xenopus laevis, Triticum aestivum, Oryctolagus cuniculus, )

IHC (Mus musculus)

co-IP (Xenopus laevis)

IF (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain images

ABclonal:Western blot - HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)}

Western blot - HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)

Western blot analysis of extracts of Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain antibody (AS063) at 1:5000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Rabbit. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Rabbit. (Distance between topics and target gene indicate popularity.) Goat Anti-Rabbit

* Data provided by citexs.com, for reference only.

Publishing research using AS063? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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