Products > Catalog Antibodies > Secondary Antibodies

HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)

Publications (22) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:Western blot - HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)

Western blot analysis of extracts of Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain antibody (AS063) at 1:2000-1:10000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameHRP-conjugated Goat Anti-Rabbit IgG Heavy Chain
Catalog No.AS063
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW55KDa
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000-10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRabbit IgG, Mouse IgG
Cellular location
Customer validation

WB(Sus scrofa, Mus musculus, Homo sapiens, Rattus norvegicus, Xenopus laevis, Triticum aestivum)

IHC(Mus musculus)

co-IP(Xenopus laevis)

IF(Homo sapiens)

    ABclonal:Western blot - HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)}

    Western blot - HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)

    Western blot analysis of extracts of Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain antibody (AS063) at 1:2000-1:10000 dilution.
    Lysates/proteins: 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

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