Products > Catalog Antibodies > Secondary Antibodies

HRP Goat Anti-Rabbit IgG (H+L) (AS014)

Publications (442) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:HRP Goat Anti-Rabbit IgG (H+L)
ABclonal:Western blot - HRP Goat Anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of extracts of HeLa cells, using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameHRP Goat Anti-Rabbit IgG (H+L)
Catalog No.AS014
Host speciesGoat
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionELISA 1:5000 - 1:10000
WB 1:2000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples
Cellular location
Customer validation

WB(Homo sapiens, Brachyura, Drosophila, Mus musculus, Rattus norvegicus, Oryza sativa, Crassostrea gigas, Saccharomyces cerevisiae, Caenorhabditis Elegans, Sus scrofa, Gallus gallus, Oyster, Cavia porcellus, Squaliobarbus curriculus, Oryctolagus cuniculus, Arabidopsis thaliana, M. truncatula, Malus micromalus, Tegillarca granosa, Zea mays, Drosophila melanogaster, Populus davidiana×P.bolleana, Abalone, Riemerella anatipestifer, Spodoptera frugiperda, Giardia duodenalis, Ictalurus punctatus, Triticum aestivum L., Eospalax cansus, Thale cress, Solanum tuberosum, Cryptosporidium parvum, broiler embryos, Ctenopharyngodon idellus, fenugreek, Toxoplasma gondii, Danio rerio, Blood clams, coli strains, Cyprinus carpio, Escherichia coli)

IHC(Mus musculus, Homo sapiens)

IHC(Homo sapiens, Scophthalmus maximus, Mus musculus, Rattus norvegicus)

ICC(Rattus norvegicus)

(Mus musculus)

ICC(Mus musculus)

(Mus musculus)

(Mus musculus)

IP(Mus musculus)

IF(Mus musculus)

IF(Homo sapiens, Rattus norvegicus, Gallus gallus)

ELISA(Grass carp)

IHC(Rattus norvegicus, Mus musculus)

IF(Homo sapiens)

ELISA(Rattus norvegicus)

    ABclonal:Western blot - HRP Goat Anti-Rabbit IgG (H+L) (AS014)}

    Western blot - HRP Goat Anti-Rabbit IgG (H+L) (AS014)

    Western blot analysis of extracts of HeLa cells, using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
    Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.

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