Products > Catalog Antibodies > Secondary Antibodies

HRP Donkey Anti-Rabbit IgG (H+L) (AS038)

Publications (4) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:Western blot - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)

Western blot analysis of extracts of HeLa cells, using β-actin antibody as the primary antibody.
Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (AS038) at 1:2000-1:30000 dilution
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)

Immunohistochemistry of paraffin-embedded Human liver (primary antibody is acox1) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)

Immunohistochemistry of paraffin-embedded Human liver (the primary antibody is acox1, and the control secondary antibody is stained) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameHRP Donkey Anti-Rabbit IgG (H+L)
Catalog No.AS038
Host speciesDonkey
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW42KDa
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:5000 - 1:100000
  • IHC 1:100-1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular location
Customer validation

WB(Mus musculus, Homo sapiens, Chlorocebus aethiops )

IHC(Mus musculus)

    ABclonal:Western blot - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)}

    Western blot - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)

    Western blot analysis of extracts of HeLa cells, using β-actin antibody as the primary antibody.
    Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (AS038) at 1:2000-1:30000 dilution
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)}

    Immunohistochemistry - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)

    Immunohistochemistry of paraffin-embedded Human liver (primary antibody is acox1) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)}

    Immunohistochemistry - HRP Donkey Anti-Rabbit IgG (H+L) (AS038)

    Immunohistochemistry of paraffin-embedded Human liver (the primary antibody is acox1, and the control secondary antibody is stained) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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