Product Type > Antibodies > Tag and Loading Control Antibodies

HRP-conjugated GAPDH Mouse mAb (AC029)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

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Overview

Product name HRP-conjugated GAPDH Mouse mAb
Catalog No. AC029
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant protein of human GAPDH
Sequence Email for sequence
Gene ID 2597
Swiss prot P04406
Synonyms G3PD; GAPD; HEL-S-162eP; HRP-conjugated GAPDH
Calculated MW 36kDa
Observed MW 36kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:3000 - 1:10000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, HepG2, MCF-7, Mouse kidney, Mouse lung, Rat liver
Cellular location Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region

Research Area

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Related Targets

Based on existing publications, the following genes are closely related to research on GAPDH. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GAPDH. (Distance between topics and target gene indicate popularity.) GAPDH

* Data provided by citexs.com, for reference only.

Publishing research using AC029? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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