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HO-1 Rabbit pAb (A1346)

Review (1)Publications (19) Datasheet

ABclonal:Western blot - HO-1 Rabbit pAb (A1346)
ABclonal:Immunohistochemistry - HO-1 Rabbit pAb (A1346)
ABclonal:Immunofluorescence - HO-1 Rabbit pAb (A1346)
Review
ABclonal: review for HO-1 Rabbit pAb(A1346)

Overview

Product nameHO-1 Rabbit pAb
Catalog No.A1346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human HO-1 (NP_002124.1).
SequenceMERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM
Gene ID3162
Swiss protP09601
SynonymsHMOX1; HMOX1D; HO-1; HSP32; bK286B10
Calculated MW32kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen, Mouse kidney, Rat kidney
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein
Customer validation

(Mouse liver)

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Struthio camelus)

ABclonal:Western blot - HO-1 Rabbit pAb (A1346) }

Western blot - HO-1 Rabbit pAb (A1346)

Western blot analysis of extracts of various cell lines, using HO-1 antibody (A1346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - HO-1 Rabbit pAb (A1346) }

Immunohistochemistry - HO-1 Rabbit pAb (A1346)

Immunohistochemistry of paraffin-embedded human liver using HO-1 antibody (A1346) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - HO-1 Rabbit pAb (A1346) }

Immunofluorescence - HO-1 Rabbit pAb (A1346)

Immunofluorescence analysis of HeLa cells using HO-1 Polyclonal Antibody (A1346) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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