Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

hnRNP U Mouse mAb (A9907)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - hnRNP U Mouse mAb (A9907)

Western blot analysis of various lysates using hnRNP U Mouse mAb (A9907) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name hnRNP U Mouse mAb
Catalog No. A9907
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14.

Immunogen information

Immunogen Recombinant protein of human hnRNP U
Sequence Email for sequence
Gene ID 3192
Swiss prot Q00839
Synonyms SAFA; DEE54; HNRPU; SAF-A; U21.1; pp120; EIEE54; GRIP120; hnRNP U; HNRNPU-AS1
Calculated MW 91kDa
Observed MW 130kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples K-562, Jurkat, HeLa, A375, Raji, SKOV3
Cellular location Cell surface, Cytoplasm, Nucleus
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

hnRNP U Mouse mAb images

ABclonal:Western blot - hnRNP U Mouse mAb (A9907)}

Western blot - hnRNP U Mouse mAb (A9907)

Western blot analysis of various lysates using hnRNP U Mouse mAb (A9907) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on HNRNPU. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HNRNPU. (Distance between topics and target gene indicate popularity.) HNRNPU

* Data provided by citexs.com, for reference only.

Publishing research using A9907? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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