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HMOX1 Polyclonal Antibody (A1346)

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ABclonal:Western blot - HMOX1 antibody (A1346)

Overview

Product nameHMOX1 Polyclonal Antibody
Catalog No.A1346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
ImmunogenRecombinant protein of human HMOX1
SequenceEmail for sequence
Gene ID3162
Swiss protP09601
SynonymsbK286B10; HMOX1D; HO-1; HSP32
Calculated MW32kDa
Observed MW33kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Mouse spleen
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein
Customer validation

WB(Mouse liver, Rat)

ABclonal:Western blot - HMOX1 antibody (A1346)}

Western blot - HMOX1 antibody (A1346)

Western blot analysis of extracts of various cell lines, using HMOX1 antibody (A1346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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