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HMGCR Rabbit pAb (A1633)

Publications (3) Datasheet

ABclonal:Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of extracts of Rat brain, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of extracts of Mouse spleen, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry of paraffin-embedded human small intestine using HMGCR antibody (A1633) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry of paraffin-embedded human uterine cancer using HMGCR antibody (A1633) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of C6 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of NIH-3T3 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of U-2 OS cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameHMGCR Rabbit pAb
Catalog No.A1633
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1).
SequenceARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKTA
Gene ID3156
Swiss protP04035
SynonymsHMGCR; LDLCQ3; HMGCR
Calculated MW92kDa/97kDa/99kDa
Observed MW97KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:4000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen, Rat brain
Cellular locationEndoplasmic reticulum membrane, Multi-pass membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - HMGCR Rabbit pAb (A1633) }

Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of extracts of Rat brain, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - HMGCR Rabbit pAb (A1633) }

Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of extracts of Mouse spleen, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633) }

Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry of paraffin-embedded human small intestine using HMGCR antibody (A1633) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633) }

Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry of paraffin-embedded human uterine cancer using HMGCR antibody (A1633) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633) }

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of C6 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633) }

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of NIH-3T3 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633) }

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of U-2 OS cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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