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HMGCR Rabbit pAb (A1633)

Publications (2) Datasheet

ABclonal:Western blot - HMGCR Polyclonal Antibody (A1633)
ABclonal:Immunohistochemistry - HMGCR Polyclonal Antibody (A1633)
ABclonal:Immunohistochemistry - HMGCR Polyclonal Antibody (A1633)

Overview

Product nameHMGCR Rabbit pAb
Catalog No.A1633
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1).
SequenceARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKTA
Gene ID3156
Swiss protP04035
SynonymsHMGCR; LDLCQ3; HMGCR
Calculated MW92kDa/97kDa/99kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:4000IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, HeLa, SH-SY5Y, BT-474, Mouse spleen, Rat liver
Cellular locationEndoplasmic reticulum membrane, Multi-pass membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - HMGCR Polyclonal Antibody (A1633) }

Western blot - HMGCR Polyclonal Antibody (A1633)

Western blot analysis of extracts of various cell lines, using HMGCR antibody (A1633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - HMGCR Polyclonal Antibody (A1633) }

Immunohistochemistry - HMGCR Polyclonal Antibody (A1633)

Immunohistochemistry of paraffin-embedded human small intestine using HMGCR antibody (A1633) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - HMGCR Polyclonal Antibody (A1633) }

Immunohistochemistry - HMGCR Polyclonal Antibody (A1633)

Immunohistochemistry of paraffin-embedded human uterine cancer using HMGCR antibody (A1633) at dilution of 1:200 (40x lens).

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