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HIF1α Rabbit pAb (A7684)

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ABclonal:Western blot - HIF1α Rabbit pAb (A7684)
ABclonal: review for HIF1α Rabbit pAb(A7684)


Product nameHIF1α Rabbit pAb
Catalog No.A7684
Host speciesRabbit
Purification methodAffinity purification
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
ImmunogenA synthetic peptide of human HIF1α
SequenceEmail for sequence
Gene ID3091
Swiss protQ16665
SynonymsHIF1A; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78
Calculated MW82kDa/92kDa/95kDa
Observed MW125kDa
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHepG2
Cellular locationCytoplasm, Nucleus, Nucleus speckle
Customer validation

(PANC-1 cells)

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

ABclonal:Western blot - HIF1α Rabbit pAb (A7684) }

Western blot - HIF1α Rabbit pAb (A7684)

Western blot analysis of extracts of HepG2 cells, using HIF1α antibody (A7684) at 1:1000 dilution.HepG2 cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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