Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

HEBP2 Rabbit pAb (A16504)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - HEBP2 Rabbit pAb (A16504)

Western blot analysis of various lysates using HEBP2 Rabbit pAb (A16504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - HEBP2 Rabbit pAb (A16504)

Immunohistochemistry analysis of HEBP2 in paraffin-embedded Rat spleen using HEBP2 Rabbit pAb (A16504) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HEBP2 Rabbit pAb (A16504)

Immunohistochemistry analysis of HEBP2 in paraffin-embedded Mouse kidney using HEBP2 Rabbit pAb (A16504) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - HEBP2 Rabbit pAb (A16504)

Immunofluorescence analysis of C6 cells using HEBP2 Rabbit pAb (A16504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HEBP2 Rabbit pAb (A16504)

Immunofluorescence analysis of L929 cells using HEBP2 Rabbit pAb (A16504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HEBP2 Rabbit pAb (A16504)

Immunofluorescence analysis of U-2 OS cells using HEBP2 Rabbit pAb (A16504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name HEBP2 Rabbit pAb
Catalog No. A16504
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is found predominately in the cytoplasm, where it plays a role in the collapse of mitochondrial membrane potential (MMP) prior to necrotic cell death. The encoded protein enhances outer and inner mitochondrial membrane permeabilization, especially under conditions of oxidative stress.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human HEBP2 (NP_055135.1).
Sequence MAEPLQPDPGAAEDAAAQAVETPGWKAPEDAGPQPGSYEIRHYGPAKWVSTSVESMDWDSAIQTGFTKLNSYIQGKNEKEMKIKMTAPVTSYVEPGSGPFSESTITISLYIPSEQQFDPPRPLESDVFIEDRAEMTVFVRSFDGFSSAQKNQEQLLTLASILREDGKVFDEKVYYTAGYNSPVKLLNRNNEVWLIQKNEPTKENE
Gene ID 23593
Swiss prot Q9Y5Z4
Synonyms PP23; SOUL; C6orf34; C6ORF34B; HEBP2
Calculated MW 23kDa
Observed MW 25kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples COS-7, Mouse lung
Cellular location Cytoplasm, Mitochondrion

Documents

Certificate of Compliance

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Lot number

HEBP2 Rabbit pAb images

ABclonal:Western blot - HEBP2 Rabbit pAb (A16504)}

Western blot - HEBP2 Rabbit pAb (A16504)

Western blot analysis of various lysates using HEBP2 Rabbit pAb (A16504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - HEBP2 Rabbit pAb (A16504)}

Immunohistochemistry - HEBP2 Rabbit pAb (A16504)

Immunohistochemistry analysis of HEBP2 in paraffin-embedded Rat spleen using HEBP2 Rabbit pAb (A16504) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HEBP2 Rabbit pAb (A16504)}

Immunohistochemistry - HEBP2 Rabbit pAb (A16504)

Immunohistochemistry analysis of HEBP2 in paraffin-embedded Mouse kidney using HEBP2 Rabbit pAb (A16504) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - HEBP2 Rabbit pAb (A16504)}

Immunofluorescence - HEBP2 Rabbit pAb (A16504)

Immunofluorescence analysis of C6 cells using HEBP2 Rabbit pAb (A16504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HEBP2 Rabbit pAb (A16504)}

Immunofluorescence - HEBP2 Rabbit pAb (A16504)

Immunofluorescence analysis of L929 cells using HEBP2 Rabbit pAb (A16504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HEBP2 Rabbit pAb (A16504)}

Immunofluorescence - HEBP2 Rabbit pAb (A16504)

Immunofluorescence analysis of U-2 OS cells using HEBP2 Rabbit pAb (A16504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on HEBP2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HEBP2. (Distance between topics and target gene indicate popularity.) HEBP2

* Data provided by citexs.com, for reference only.

Publishing research using A16504? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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