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GSK3β Rabbit pAb (A2081)

Reviews (2)Publications (6) Datasheet

ABclonal:Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of extracts of mouse brain, using GSK3β antibody (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry of paraffin-embedded human mammary cancer using GSK3β antibody (A2081) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry of paraffin-embedded human liver cancer using GSK3β antibody (A2081) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry of paraffin-embedded mouse spleen using GSK3β antibody (A2081) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - GSK3β Rabbit pAb (A2081)

Immunofluorescence analysis of C6 cells using GSK3β antibody (A2081) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A2081)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug GSK3β antibody (A2081). Western blot was performed from the immunoprecipitate using GSK3β antibody (A2081) at a dilition of 1:1000.

Review
ABclonal: review for GSK3β Rabbit pAb(A2081)
Review
ABclonal: review for GSK3β Rabbit pAb(A2081)

Overview

Product nameGSK3β Rabbit pAb
Catalog No.A2081
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide of human GSK3β
SequenceEmail for sequence
Gene ID2932
Swiss protP49841
SynonymsGSK3B; gsk-3β
Calculated MW46kDa/48kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMouse brain
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

(Human chronic myeloid leukemia cells)

WB(Homo sapiens, Rattus norvegicus)

IF(Homo sapiens)

ICC(Homo sapiens)

ABclonal:Western blot - GSK3β Rabbit pAb (A2081) }

Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of extracts of mouse brain, using GSK3β antibody (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081) }

Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry of paraffin-embedded human mammary cancer using GSK3β antibody (A2081) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081) }

Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry of paraffin-embedded human liver cancer using GSK3β antibody (A2081) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081) }

Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry of paraffin-embedded mouse spleen using GSK3β antibody (A2081) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - GSK3β Rabbit pAb (A2081) }

Immunofluorescence - GSK3β Rabbit pAb (A2081)

Immunofluorescence analysis of C6 cells using GSK3β antibody (A2081) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A2081) }

Immunoprecipitation - GSK3β Rabbit pAb (A2081)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug GSK3β antibody (A2081). Western blot was performed from the immunoprecipitate using GSK3β antibody (A2081) at a dilition of 1:1000.

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