Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GSK3β Rabbit pAb (A2081)

Reviews (2) Publications (28) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of lysates from mouse brain, using GSK3β Rabbit pAb (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of various lysates using GSK3β Rabbit pAb (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of lysates from 293T cells, using GSK3β Rabbit pAb (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry analysis of GSK3β in paraffin-embedded human mammary cancer using GSK3β Rabbit pAb (A2081) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry analysis of GSK3β in paraffin-embedded human liver cancer using GSK3β Rabbit pAb (A2081) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry analysis of GSK3β in paraffin-embedded mouse spleen using GSK3β Rabbit pAb (A2081) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GSK3β Rabbit pAb (A2081)

Immunofluorescence analysis of C6 cells using GSK3β Rabbit pAb (A2081) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A2081)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg GSK3β antibody (A2081). Western blot was performed from the immunoprecipitate using GSK3β antibody (A2081) at a dilution of 1:1000.

Review
ABclonal: review for GSK3β Rabbit pAb(A2081)
Review
ABclonal: review for GSK3β Rabbit pAb(A2081)

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Overview

Product name GSK3β Rabbit pAb
Catalog No. A2081
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 350-420 of human GSK3β (NP_001139628.1).
Sequence LPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST
Gene ID 2932
Swiss prot P49841
Synonyms GSK3B; gsk-3β; GSK3β
Calculated MW 47kDa
Observed MW 46kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples Mouse brain
Cellular location Cell membrane, Cytoplasm, Nucleus
Customer validation

WB (Human chronic myeloid leukemia cellsRattus norvegicus, Homo sapiens, Rattus norvegicus, Mus musculus, bird, Gallus gallus, Cyprinus carpio, Capra hircus)

IF (Human chronic myeloid leukemia cellsHomo sapiens)

ICC (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

GSK3β Rabbit pAb images

ABclonal:Western blot - GSK3β Rabbit pAb (A2081)}

Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of lysates from mouse brain, using GSK3β Rabbit pAb (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - GSK3β Rabbit pAb (A2081)}

Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of various lysates using GSK3β Rabbit pAb (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - GSK3β Rabbit pAb (A2081)}

Western blot - GSK3β Rabbit pAb (A2081)

Western blot analysis of lysates from 293T cells, using GSK3β Rabbit pAb (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)}

Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry analysis of GSK3β in paraffin-embedded human mammary cancer using GSK3β Rabbit pAb (A2081) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)}

Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry analysis of GSK3β in paraffin-embedded human liver cancer using GSK3β Rabbit pAb (A2081) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GSK3β Rabbit pAb (A2081)}

Immunohistochemistry - GSK3β Rabbit pAb (A2081)

Immunohistochemistry analysis of GSK3β in paraffin-embedded mouse spleen using GSK3β Rabbit pAb (A2081) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GSK3β Rabbit pAb (A2081)}

Immunofluorescence - GSK3β Rabbit pAb (A2081)

Immunofluorescence analysis of C6 cells using GSK3β Rabbit pAb (A2081) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A2081)}

Immunoprecipitation - GSK3β Rabbit pAb (A2081)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg GSK3β antibody (A2081). Western blot was performed from the immunoprecipitate using GSK3β antibody (A2081) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on GSK3B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GSK3B. (Distance between topics and target gene indicate popularity.) GSK3B

* Data provided by citexs.com, for reference only.

Publishing research using A2081? Please let us know so that we can cite the reference in this datasheet.

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