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GSK3β Rabbit pAb (A16868)

Publications (2) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A16868)

Immunoprecipitation analysis of 200ug extracts of LO2 cells using 3ug GSK3β antibody (A16868). Western blot was performed from the immunoprecipitate using GSK3β antibody (A16868) at a dilition of 1:1000.

Overview

Product nameGSK3β Rabbit pAb
Catalog No.A16868
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 350-420 of GSK3β (NP_001139628.1).
SequenceLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST
Gene ID2932
Swiss protP49841
SynonymsGSK3B; gsk-3β
Calculated MW46kDa/48kDa
Observed MW48KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samples293T, NIH/3T3, Mouse testis, Rat testis
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A16868)}

Immunoprecipitation - GSK3β Rabbit pAb (A16868)

Immunoprecipitation analysis of 200ug extracts of LO2 cells using 3ug GSK3β antibody (A16868). Western blot was performed from the immunoprecipitate using GSK3β antibody (A16868) at a dilition of 1:1000.

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