Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GSK3α Rabbit pAb (A0645)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - GSK3α Rabbit pAb (A0645)

Western blot analysis of extracts of various cell lines, using GSK3α antibody (A0645) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GSK3α Rabbit pAb (A0645)

Immunofluorescence analysis of NIH/3T3 cells using GSK3α antibody (A0645) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Overview

Product name GSK3α Rabbit pAb
Catalog No. A0645
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 409-483 of human GSK3α (NP_063937.2).
Sequence LGTQLPNNRPLPPLFNFSAGELSIQPSLNAILIPPHLRSPAGTTTLTPSSQALTETPTSSDWQSTDATPTLTNSS
Gene ID 2931
Swiss prot P49840
Synonyms GSK3 alpha; GSK3A; GSK3α
Calculated MW 51kDa
Observed MW 51kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples U-87MG, A-431, HeLa, B-cell, Mouse liver
Cellular location apical dendrite, axon, beta-catenin destruction complex, cytoplasm, cytosol, mitochondrion, nucleus, postsynapse, proximal dendrite
Customer validation

WB (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

GSK3α Rabbit pAb images

ABclonal:Western blot - GSK3α Rabbit pAb (A0645)}

Western blot - GSK3α Rabbit pAb (A0645)

Western blot analysis of extracts of various cell lines, using GSK3α antibody (A0645) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - GSK3α Rabbit pAb (A0645)}

Immunofluorescence - GSK3α Rabbit pAb (A0645)

Immunofluorescence analysis of NIH/3T3 cells using GSK3α antibody (A0645) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on GSK3A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GSK3A. (Distance between topics and target gene indicate popularity.) GSK3A

* Data provided by citexs.com, for reference only.

Publishing research using A0645? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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