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GSK3B Polyclonal Antibody (A2081)

Reviews (2)Publications (4) Datasheet

ABclonal:Western blot - GSK3B Polyclonal Antibody (A2081)
ABclonal:Immunohistochemistry - GSK3B Polyclonal Antibody (A2081)
ABclonal:Immunohistochemistry - GSK3B Polyclonal Antibody (A2081)
ABclonal:Immunohistochemistry - GSK3B Polyclonal Antibody (A2081)
ABclonal:Immunofluorescence - GSK3B Polyclonal Antibody (A2081)
Review
ABclonal: review for GSK3B Polyclonal Antibody(A2081)
Review
ABclonal: review for GSK3B Polyclonal Antibody(A2081)

Overview

Product nameGSK3B Polyclonal Antibody
Catalog No.A2081
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide of human GSK3B
SequenceEmail for sequence
Gene ID2932
Swiss protP49841
SynonymsGSK3B; glycogen synthase kinase-3 beta
Calculated MW46kDa/48kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMouse brain
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

(, )

WB(Human, Rat)

ICC(Human)

ABclonal:Western blot - GSK3B Polyclonal Antibody (A2081) }

Western blot - GSK3B Polyclonal Antibody (A2081)

Western blot analysis of extracts of mouse brain, using GSK3B antibody (A2081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GSK3B Polyclonal Antibody (A2081) }

Immunohistochemistry - GSK3B Polyclonal Antibody (A2081)

Immunohistochemistry of paraffin-embedded human mammary cancer using GSK3B antibody (A2081) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - GSK3B Polyclonal Antibody (A2081) }

Immunohistochemistry - GSK3B Polyclonal Antibody (A2081)

Immunohistochemistry of paraffin-embedded human liver cancer using GSK3B antibody (A2081) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - GSK3B Polyclonal Antibody (A2081) }

Immunohistochemistry - GSK3B Polyclonal Antibody (A2081)

Immunohistochemistry of paraffin-embedded mouse spleen using GSK3B antibody (A2081) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - GSK3B Polyclonal Antibody (A2081) }

Immunofluorescence - GSK3B Polyclonal Antibody (A2081)

Immunofluorescence analysis of C6 cells using GSK3B antibody (A2081) at dilution of 1:100. Blue: DAPI for nuclear staining.

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