Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

GSK3β Rabbit mAb (A10877)

Review (1) Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - GSK3β Rabbit mAb (A10877)

Western blot analysis of extracts of various cell lines, using GSK3β antibody (A10877).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Review
ABclonal: review for GSK3β Rabbit mAb(A10877)

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Overview

Product name GSK3β Rabbit mAb
Catalog No. A10877
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

Immunogen information

Immunogen Recombinant protein of human GSK3β
Sequence Email for sequence
Gene ID 2932
Swiss prot P49841
Synonyms GSK3B; gsk-3β; GSK3β
Calculated MW 47kDa
Observed MW 47kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:3000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, 293T, NIH/3T3, PC-12
Cellular location Cell membrane, Cytoplasm, Nucleus
Customer validation

WB (BGC823Homo sapiens)

Research Area

GSK3β Rabbit mAb images

ABclonal:Western blot - GSK3β Rabbit mAb (A10877)}

Western blot - GSK3β Rabbit mAb (A10877)

Western blot analysis of extracts of various cell lines, using GSK3β antibody (A10877).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on GSK3B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GSK3B. (Distance between topics and target gene indicate popularity.) GSK3B

* Data provided by citexs.com, for reference only.

Publishing research using A10877? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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