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GRK1 Rabbit pAb (A6497)

Reviews (3)Publications (2) Datasheet

ABclonal:Western blot - GRK1 Rabbit pAb (A6497)

Western blot analysis of extracts of Y79 cells, using GRK1 antibody (A6497).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - GRK1 Rabbit pAb (A6497)

Immunofluorescence analysis of rat retina using GRK1 Polyclonal Antibody (A6497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GRK1 Rabbit pAb (A6497)

Immunofluorescence analysis of mouse retina using GRK1 Polyclonal Antibody (A6497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for GRK1 Rabbit pAb(A6497)
Review
ABclonal: review for GRK1 Rabbit pAb(A6497)
Review
ABclonal: review for GRK1 Rabbit pAb(A6497)

Overview

Product nameGRK1 Rabbit pAb
Catalog No.A6497
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1).
SequenceMDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKKLFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVEIQDGLFQPLLQATLAHLGQA
Gene ID6011
Swiss protQ15835
SynonymsGRK1; GPRK1; RHOK; RK
Calculated MW63kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesY79
Cellular locationLipid-anchor, Membrane
Customer validation

(Zebrafish)

WB(Danio rerio)

IF(Danio rerio)

IHC(Danio rerio)

ABclonal:Western blot - GRK1 Rabbit pAb (A6497) }

Western blot - GRK1 Rabbit pAb (A6497)

Western blot analysis of extracts of Y79 cells, using GRK1 antibody (A6497).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - GRK1 Rabbit pAb (A6497) }

Immunofluorescence - GRK1 Rabbit pAb (A6497)

Immunofluorescence analysis of rat retina using GRK1 Polyclonal Antibody (A6497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GRK1 Rabbit pAb (A6497) }

Immunofluorescence - GRK1 Rabbit pAb (A6497)

Immunofluorescence analysis of mouse retina using GRK1 Polyclonal Antibody (A6497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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