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GPX4 Rabbit mAb (A11243)

Datasheet

ABclonal:Western blot - GPX4 Rabbit mAb (A11243)
ABclonal:Immunoprecipitation - GPX4 Rabbit mAb (A11243)

Overview

Product nameGPX4 Rabbit mAb
Catalog No.A11243
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]
ImmunogenA synthesized peptide derived from human GPX4
SequenceEmail for sequence
Gene ID2879
Swiss protP36969
SynonymsGPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx
Calculated MW17kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesPC-3, Jurkat, Mouse kidney, Mouse heart, Rat kidney
Cellular locationCytoplasm, Mitochondrion
ABclonal:Western blot - GPX4 Rabbit mAb (A11243) }

Western blot - GPX4 Rabbit mAb (A11243)

Western blot analysis of extracts of various cell lines, using GPX4 Rabbit mAb (A11243) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - GPX4 Rabbit mAb (A11243) }

Immunoprecipitation - GPX4 Rabbit mAb (A11243)

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug GPX4 antibody (A11243). Western blot was performed from the immunoprecipitate using GPX4 antibody (A11243) at a dilition of 1:1000.

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