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GPER1 Polyclonal Antibody (A10217)

Publication (1) Datasheet

ABclonal:Western blot - GPER1 Polyclonal Antibody (A10217)
ABclonal:Immunofluorescence - GPER1 Polyclonal Antibody (A10217)
ABclonal:Immunofluorescence - GPER1 Polyclonal Antibody (A10217)
ABclonal:Immunofluorescence - GPER1 Polyclonal Antibody (A10217)

Overview

Product nameGPER1 Polyclonal Antibody
Catalog No.A10217
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3, 4, 5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human GPER1 (NP_001496.1).
SequenceMDVTSQARGVGLEMYPGTAQPAAPNTTSPELNLSHPLLGTALANGTGELSEHQQYVIGLFLSCLYTIFLF
Gene ID2852
Swiss protQ99527
SynonymsGPER1; CEPR; CMKRL2; DRY12; FEG-1; GPCR-Br; GPER; GPR30; LERGU; LERGU2; LyGPR; mER; G-protein coupled estrogen receptor 1
Calculated MW42kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Mouse heart, Rat brain
Cellular locationBasolateral cell membrane, Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle membrane, Early endosome, Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus membrane, Mitochondrion membrane, Multi-pass membrane protein, Nucleus, Recycling endosome, axon, cytoskeleton, dendrite, dendritic spine membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network
Customer validation

WB(Mouse)

ABclonal:Western blot - GPER1 Polyclonal Antibody (A10217) }

Western blot - GPER1 Polyclonal Antibody (A10217)

Western blot analysis of extracts of various cell lines, using GPER1 antibody (A10217) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - GPER1 Polyclonal Antibody (A10217) }

Immunofluorescence - GPER1 Polyclonal Antibody (A10217)

Immunofluorescence analysis of C6 cells using GPER1 antibody (A10217) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GPER1 Polyclonal Antibody (A10217) }

Immunofluorescence - GPER1 Polyclonal Antibody (A10217)

Immunofluorescence analysis of NIH/3T3 cells using GPER1 antibody (A10217) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GPER1 Polyclonal Antibody (A10217) }

Immunofluorescence - GPER1 Polyclonal Antibody (A10217)

Immunofluorescence analysis of U2OS cells using GPER1 antibody (A10217) at dilution of 1:100. Blue: DAPI for nuclear staining.

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