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GOLPH2 Rabbit pAb (A7283)

Datasheet

ABclonal:Western blot - GOLPH2 Rabbit pAb (A7283)

Western blot analysis of extracts of Rat lung, using GOLPH2 antibody (A7283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - GOLPH2 Rabbit pAb (A7283)

Immunohistochemistry of paraffin-embedded rat brain using GOLPH2 Rabbit pAb (A7283) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - GOLPH2 Rabbit pAb (A7283)

Immunohistochemistry of paraffin-embedded human colon carcinoma using GOLPH2 Rabbit pAb (A7283) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - GOLPH2 Rabbit pAb (A7283)

Immunofluorescence analysis of U2OS cells using GOLPH2 Rabbit pAb (A7283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGOLPH2 Rabbit pAb
Catalog No.A7283
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 112-401 of human GOLPH2 (NP_057632.2).
SequenceTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL
Gene ID51280
Swiss protQ8NBJ4
SynonymsGOLM1; C9orf155; GOLPH2; GP73; HEL46; PSEC0257; bA379P1.3
Calculated MW44kDa/45kDa
Observed MW75KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat lung
Cellular locationGolgi apparatus, Single-pass type II membrane protein, cis-Golgi network membrane
    ABclonal:Western blot - GOLPH2 Rabbit pAb (A7283) }

    Western blot - GOLPH2 Rabbit pAb (A7283)

    Western blot analysis of extracts of Rat lung, using GOLPH2 antibody (A7283) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - GOLPH2 Rabbit pAb (A7283) }

    Immunohistochemistry - GOLPH2 Rabbit pAb (A7283)

    Immunohistochemistry of paraffin-embedded rat brain using GOLPH2 Rabbit pAb (A7283) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - GOLPH2 Rabbit pAb (A7283) }

    Immunohistochemistry - GOLPH2 Rabbit pAb (A7283)

    Immunohistochemistry of paraffin-embedded human colon carcinoma using GOLPH2 Rabbit pAb (A7283) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - GOLPH2 Rabbit pAb (A7283) }

    Immunofluorescence - GOLPH2 Rabbit pAb (A7283)

    Immunofluorescence analysis of U2OS cells using GOLPH2 Rabbit pAb (A7283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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