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GM130 Rabbit pAb (A5344)

Review (1)Publications (9) Datasheet

ABclonal:Western blot - GM130 Rabbit pAb (A5344)

Western blot analysis of extracts of various cell lines, using (A5344) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - GM130 Rabbit pAb (A5344)

Western blot analysis of extracts of Rat liver, using (A5344) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - GM130 Rabbit pAb (A5344)

Confocal immunofluorescence analysis of Hela cells using GM130 antibody (A5344) at dilution of 1:400. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - GM130 Rabbit pAb (A5344)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GM130 antibody (A5344). Western blot was performed from the immunoprecipitate using GM130 antibody (A5344) at a dilition of 1:1000.

Review
ABclonal: review for GM130 Rabbit pAb(A5344)

Overview

Product nameGM130 Rabbit pAb
Catalog No.A5344
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GM130 (NP_004477.3).
SequenceSKLAAAKKKLREYQQRNSPGVPTGAKKKKKIKNGSNPETTTSGGCHSPEDTPKDNAATLQPSDDTVLPGGVPSPGASLTSMAASQNHDADNVPNLMDETKTFSSTESLRQLSQQLNGLVCESATCVNGEGPASSANLKDLESRYQQLAVALDSSYVTNKQLNITIEKLKQQNQEITDQLEEEKKECHQKQGALREQLQVHIQTIGILVSEKAELQTALAHTQHAARQKEGESEDLASRLQYSRRRVGELERALSAVSTQQKKADRYNKELTKERDALRLEL
Gene ID2801
Swiss protQ08379
SynonymsGOLGA2; GM130; golgin A2
Calculated MW70kDa/113kDa
Observed MW130KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHepG2, HeLa, Rat liver
Cellular locationCytoplasm, Golgi apparatus, Peripheral membrane protein, cis-Golgi network membrane, cytoskeleton, spindle pole
Customer validation

(293T)

IF(Homo sapiens,Mus musculus)

WB(Homo sapiens, Mus musculus)

IF(Mus musculus)

    ABclonal:Western blot - GM130 Rabbit pAb (A5344) }

    Western blot - GM130 Rabbit pAb (A5344)

    Western blot analysis of extracts of various cell lines, using (A5344) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Western blot - GM130 Rabbit pAb (A5344) }

    Western blot - GM130 Rabbit pAb (A5344)

    Western blot analysis of extracts of Rat liver, using (A5344) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - GM130 Rabbit pAb (A5344) }

    Immunofluorescence - GM130 Rabbit pAb (A5344)

    Confocal immunofluorescence analysis of Hela cells using GM130 antibody (A5344) at dilution of 1:400. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - GM130 Rabbit pAb (A5344) }

    Immunoprecipitation - GM130 Rabbit pAb (A5344)

    Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GM130 antibody (A5344). Western blot was performed from the immunoprecipitate using GM130 antibody (A5344) at a dilition of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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