Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GNAI3 Rabbit pAb (A15674)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - GNAI3 Rabbit pAb (A15674)

Western blot analysis of extracts of various cell lines, using GNAI3 antibody (A15674) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameGNAI3 Rabbit pAb
Catalog No.A15674
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1).
SequenceMGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAAR
Gene ID2773
Swiss protP08754
SynonymsGNAI3; 87U6; ARCND1
Calculated MW40kDa
Observed MW40kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesLO2, HepG2, Mouse kidney, Mouse brain, Rat kidney
Cellular locationCell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center

Research Area

GNAI3 Rabbit pAb images

ABclonal:Western blot - GNAI3 Rabbit pAb (A15674)}

Western blot - GNAI3 Rabbit pAb (A15674)

Western blot analysis of extracts of various cell lines, using GNAI3 antibody (A15674) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on GNAI3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GNAI3. (Distance between topics and target gene indicate popularity.) GNAI3

* Data provided by citexs.com, for reference only.

Publishing research using A15674? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (2)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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