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GLUT4 Rabbit pAb (A7637)

Review (1)Publications (6) Datasheet

ABclonal:Western blot - GLUT4 Rabbit pAb (A7637)

Western blot analysis of extracts of various cell lines, using GLUT4 antibody (A7637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GLUT4 Rabbit pAb (A7637)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of Mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for GLUT4 Rabbit pAb(A7637)

Overview

Product nameGLUT4 Rabbit pAb
Catalog No.A7637
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human GLUT4 (NP_001033.1).
SequenceGPIPWFIVAELFSQGPRPAAMAVAGFSNWTSNFIIGMGFQYVAEAMGPYVFLLFAVLLLGFFIFTFLRVPETRGRTFDQISAAFHRTPSLLEQEVKPSTELEYLGPDEND
Gene ID6517
Swiss protP14672
SynonymsSLC2A4; GLUT4
Calculated MW43kDa/54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, HepG2, Mouse kidney, Mouse heart, Rat heart, Rat kidney
Cellular locationCell membrane, Cytoplasm, Endomembrane system, Multi-pass membrane protein, perinuclear region
Customer validation

(Mouse heart tissue)

WB(Homo sapiens, Mus musculus)

IHC(Mesocricetus auratus)

ABclonal:Western blot - GLUT4 Rabbit pAb (A7637) }

Western blot - GLUT4 Rabbit pAb (A7637)

Western blot analysis of extracts of various cell lines, using GLUT4 antibody (A7637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GLUT4 Rabbit pAb (A7637) }

Immunohistochemistry - GLUT4 Rabbit pAb (A7637)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637) }

Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of Mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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