Products > Catalog Antibodies > Polyclonal Antibodies

GLUT4 Rabbit pAb (A7637)

Review (1)Publications (11) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - GLUT4 Rabbit pAb (A7637)

Western blot analysis of extracts of various cell lines, using GLUT4 antibody (A7637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - GLUT4 Rabbit pAb (A7637)

Western blot analysis of extracts of various cell lines, using GLUT4 antibody (A7637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GLUT4 Rabbit pAb (A7637)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GLUT4 Rabbit pAb (A7637)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of Mouse skeletal muscle using GLUT4 antibody (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of RD cells using GLUT4 antibody (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of C2C12 cells using GLUT4 antibody (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for GLUT4 Rabbit pAb(A7637)

Overview

Product nameGLUT4 Rabbit pAb
Catalog No.A7637
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-509 of human GLUT4 (NP_001033.1).
SequenceFLLFAVLLLGFFIFTFLRVPETRGRTFDQISAAFHRTPSLLEQEVKPSTELEYLGPDEND
Gene ID6517
Swiss protP14672
SynonymsSLC2A4; GLUT4
Calculated MW43kDa/54kDa
Observed MW55KDs
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, RD, Jurkat, NIH/3T3, H9C2
Cellular locationCell membrane, Cytoplasm, Endomembrane system, Multi-pass membrane protein, perinuclear region
Customer validation

(Mouse heart tissue)

WB(Homo sapiens, Mus musculus)

IHC(Mesocricetus auratus)

ABclonal:Western blot - GLUT4 Rabbit pAb (A7637)}

Western blot - GLUT4 Rabbit pAb (A7637)

Western blot analysis of extracts of various cell lines, using GLUT4 antibody (A7637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - GLUT4 Rabbit pAb (A7637)}

Western blot - GLUT4 Rabbit pAb (A7637)

Western blot analysis of extracts of various cell lines, using GLUT4 antibody (A7637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GLUT4 Rabbit pAb (A7637)}

Immunohistochemistry - GLUT4 Rabbit pAb (A7637)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GLUT4 Rabbit pAb (A7637)}

Immunohistochemistry - GLUT4 Rabbit pAb (A7637)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GLUT4 Rabbit pAb (A7637) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637)}

Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of Mouse skeletal muscle using GLUT4 antibody (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637)}

Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of RD cells using GLUT4 antibody (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GLUT4 Rabbit pAb (A7637)}

Immunofluorescence - GLUT4 Rabbit pAb (A7637)

Immunofluorescence analysis of C2C12 cells using GLUT4 antibody (A7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Submit an review for A7637

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A7637 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A7637? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order