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GLUT1/SLC2A1 Rabbit pAb (A6982)

Publications (6) Datasheet

ABclonal:Western blot - GLUT1/SLC2A1 Rabbit pAb (A6982)

Western blot analysis of extracts of various cell lines, using GLUT1/SLC2A1 Rabbit pAb (A6982) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982)

Immunohistochemistry of paraffin-embedded Human placenta using GLUT1/SLC2A1 antibody (A6982) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982)

Immunohistochemistry of paraffin-embedded Rat placenta using GLUT1/SLC2A1 antibody (A6982) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982)

Immunohistochemistry of paraffin-embedded Mouse placenta using GLUT1/SLC2A1 antibody (A6982) at dilution of 1:100 (40x lens).

Overview

Product nameGLUT1/SLC2A1 Rabbit pAb
Catalog No.A6982
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-310 of human GLUT1/SLC2A1 (NP_006507.2).
SequenceSPRFLLINRNEENRAKSVLKKLRGTADVTHDLQEMKEESRQMMREKKVTILELFRSPAYRQPILIAVVLQLSQQLSGINAVFYYSTSIFEKAGVQQPVYAT
Gene ID6513
Swiss protP11166
SynonymsSLC2A1; CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN
Calculated MW54kDa
Observed MW45-60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T
Cellular locationCell membrane, Melanosome, Multi-pass membrane protein
Customer validation

WB(Rana sylvatica, Homo sapiens, Mus musculus)

ABclonal:Western blot - GLUT1/SLC2A1 Rabbit pAb (A6982) }

Western blot - GLUT1/SLC2A1 Rabbit pAb (A6982)

Western blot analysis of extracts of various cell lines, using GLUT1/SLC2A1 Rabbit pAb (A6982) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982) }

Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982)

Immunohistochemistry of paraffin-embedded Human placenta using GLUT1/SLC2A1 antibody (A6982) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982) }

Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982)

Immunohistochemistry of paraffin-embedded Rat placenta using GLUT1/SLC2A1 antibody (A6982) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982) }

Immunohistochemistry - GLUT1/SLC2A1 Rabbit pAb (A6982)

Immunohistochemistry of paraffin-embedded Mouse placenta using GLUT1/SLC2A1 antibody (A6982) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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