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GLP1R Rabbit pAb (A8547)

Publications (6) Datasheet

ABclonal:Western blot - GLP1R Rabbit pAb (A8547)

Western blot analysis of extracts of various cell lines, using GLP1R antibody (A8547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - GLP1R Rabbit pAb (A8547)

Immunofluorescence analysis of L929 cells using GLP1R antibody (A8547) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameGLP1R Rabbit pAb
Catalog No.A8547
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-116 of human GLP1R (NP_002053.3).
SequenceGPRPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNS
Gene ID2740
Swiss protP43220
SynonymsGLP1R; GLP-1; GLP-1-R; GLP-1R
Calculated MW53kDa
Observed MW53kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse lung, Rat stomach, Rat lung
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - GLP1R Rabbit pAb (A8547) }

Western blot - GLP1R Rabbit pAb (A8547)

Western blot analysis of extracts of various cell lines, using GLP1R antibody (A8547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - GLP1R Rabbit pAb (A8547) }

Immunofluorescence - GLP1R Rabbit pAb (A8547)

Immunofluorescence analysis of L929 cells using GLP1R antibody (A8547) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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