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GLI1 Rabbit pAb (A14675)

Publications (6) Datasheet

ABclonal:Western blot - GLI1 Rabbit pAb (A14675)

Western blot analysis of extracts of Mouse brain, using GLI1 antibody (A14675) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - GLI1 Rabbit pAb (A14675)

Immunohistochemistry of paraffin-embedded human breast cancer using GLI1 Rabbit pAb (A14675) at dilution of 1:150 (40x lens).

ABclonal:Immunohistochemistry - GLI1 Rabbit pAb (A14675)

Immunohistochemistry of paraffin-embedded mouse uterus using GLI1 Rabbit pAb (A14675) at dilution of 1:150 (40x lens).

ABclonal:Immunohistochemistry - GLI1 Rabbit pAb (A14675)

Immunohistochemistry of paraffin-embedded rat lung using GLI1 Rabbit pAb (A14675) at dilution of 1:150 (40x lens).

ABclonal:Immunofluorescence - GLI1 Rabbit pAb (A14675)

Immunofluorescence analysis of HeLa cells using GLI1 antibody (A14675) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GLI1 Rabbit pAb (A14675)

Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody (A14675) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameGLI1 Rabbit pAb
Catalog No.A14675
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1).
SequenceEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESR
Gene ID2735
Swiss protP08151
SynonymsGLI1; GLI
Calculated MW104kDa/113kDa/117kDa
Observed MW150KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain
Cellular locationCytoplasm, Nucleus, Nucleus
Customer validation

IHC(Homo sapiens, Mus musculus)

WB(Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - GLI1 Rabbit pAb (A14675) }

Western blot - GLI1 Rabbit pAb (A14675)

Western blot analysis of extracts of Mouse brain, using GLI1 antibody (A14675) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - GLI1 Rabbit pAb (A14675) }

Immunohistochemistry - GLI1 Rabbit pAb (A14675)

Immunohistochemistry of paraffin-embedded human breast cancer using GLI1 Rabbit pAb (A14675) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - GLI1 Rabbit pAb (A14675) }

Immunohistochemistry - GLI1 Rabbit pAb (A14675)

Immunohistochemistry of paraffin-embedded mouse uterus using GLI1 Rabbit pAb (A14675) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - GLI1 Rabbit pAb (A14675) }

Immunohistochemistry - GLI1 Rabbit pAb (A14675)

Immunohistochemistry of paraffin-embedded rat lung using GLI1 Rabbit pAb (A14675) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - GLI1 Rabbit pAb (A14675) }

Immunofluorescence - GLI1 Rabbit pAb (A14675)

Immunofluorescence analysis of HeLa cells using GLI1 antibody (A14675) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GLI1 Rabbit pAb (A14675) }

Immunofluorescence - GLI1 Rabbit pAb (A14675)

Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody (A14675) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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