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GFAP Rabbit pAb (A0237)

Reviews (2)Publications (8) Datasheet

ABclonal:Western blot - GFAP Rabbit pAb (A0237)

Western blot analysis of extracts of various cell lines, using GFAP Rabbit pAb (A0237) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - GFAP Rabbit pAb (A0237)

Immunohistochemistry of paraffin-embedded rat brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - GFAP Rabbit pAb (A0237)

Immunohistochemistry of paraffin-embedded human brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of U-251 MG cells using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of rat brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of mouse brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for GFAP Rabbit pAb(A0237)
Review
ABclonal: review for GFAP Rabbit pAb(A0237)

Overview

Product nameGFAP Rabbit pAb
Catalog No.A0237
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
SequenceRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
Gene ID2670
Swiss protP14136
SynonymsALXDRD; GFAP
Calculated MW49kDa/50kDa
Observed MW50KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCytoplasm
Customer validation

(Mouse glial cells, Mouse brain)

IF(Anatinae, Rattus norvegicus, Mus musculus, Homo sapiens)

WB(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - GFAP Rabbit pAb (A0237) }

Western blot - GFAP Rabbit pAb (A0237)

Western blot analysis of extracts of various cell lines, using GFAP Rabbit pAb (A0237) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - GFAP Rabbit pAb (A0237) }

Immunohistochemistry - GFAP Rabbit pAb (A0237)

Immunohistochemistry of paraffin-embedded rat brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GFAP Rabbit pAb (A0237) }

Immunohistochemistry - GFAP Rabbit pAb (A0237)

Immunohistochemistry of paraffin-embedded human brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237) }

Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of U-251 MG cells using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237) }

Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of rat brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237) }

Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of mouse brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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